Development of microsatellite markers for Pinus maximinoiderived from microsatellite-enriched libraries
نویسندگان
چکیده
منابع مشابه
Development of microsatellite markers for Pinus maximinoi derived from microsatellite-enriched libraries
Background Integrating the use of molecular techniques in ongoing breeding can help identifying divergent genotypes to be used in controlled crosses for the development of hybrids, estimating parentage and monitoring the efficient use of genetic variation. Breeding programs carried out at EMBRAPA have established a broad population genetic base for various species of pine, from which trees with...
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Simple sequence repeats (SSRs), or microsatellites, have been isolated and characterized from numerous animal and plant species (6,7). These sequences have been shown to be highly polymorphic between individuals within populations or closely related genotypes. This, together with the fact that they can be converted into a simple polymerase chain reaction (PCR)based assay, has led to their wides...
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The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes their low efficiency or lack of optimization in the protocols can restrict their ...
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A strategy combining dual-suppression PCR and thermal asymmetric interlaced PCR was used to determine sequences flanking microsatellite regions in Pythium helicoides. The primer pairs were designed to amplify loci containing (AC)n, (GA)n, (AGC)n, (CAC)n(CAA)n, (TCA)n and (CTTT)n repeats from the P. helicoides nuclear genome. The PCR products of each primer pair, amplified from three representat...
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The taxonomic status of Pinus henryi, a rare species endemic to China, is still ambiguous. In this study, the genetic relationships among P. henryi and its congeners (P. tabulaeformis, P. tabulaeformis var. mukdensis, and P. massoniana) were revealed using multiplexed microsatellite markers, including chloroplast microsatellites, nuclear microsatellites, and expressed sequence tag microsatellit...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2011
ISSN: 1753-6561
DOI: 10.1186/1753-6561-5-s7-p2